Peptide Concentration & Dilution Calculator

Work out stock-solution concentration (mg/mL) and the aliquot volume for a target mass of lyophilised research peptide. In vitro laboratory use only.

Vial contents (mg)

Or enter another vial size (mg)

Required aliquot

Required aliquot (mcg)

Required aliquot (mg)

Solvent volume to add

Or enter a custom solvent volume (mL)

About this calculator

This free peptide calculator helps researchers work out reconstitution, concentration and sample size in the lab — including for BPC-157, TB-500, GHK-Cu, CJC-1295 and other lyophilised peptides used in in vitro studies. Enter your vial amount in mg, your solvent volume in mL and a required sample size in mcg or mg. The tool returns the stock concentration in mg/mL and the transfer volume in mL and microlitres.

Unit conversions

1 mL = 1000 µL

1 mg = 1000 mcg

1 µL = 0.001 mL

Worked examples

Example 1: 10mg peptide vial

Scenario: 10mg peptide, want 250 mcg sample

Suggested solvent volume: Add 2 mL solvent

Result: Transfer 0.05mL (50 µL) for ~250 mcg sample

Example 2: 5mg peptide vial

Scenario: 5mg peptide, want 500 mcg sample

Suggested solvent volume: Add 1 mL solvent

Result: Transfer 0.1mL (100 µL) for ~500 mcg sample

Examples by vial size

For in vitro research use only. Illustrative measurement scenarios — not human or veterinary guidance.

BPC-157 5mg

Vial: 5mg lyophilised BPC-157

Stock solution: 2 mL solvent → 2.5 mg/mL

250 mcg aliquot: transfer 0.1mL (100 µL)

TB-500 5mg

Vial: 5mg lyophilised TB-500

Stock solution: 2 mL solvent → 2.5 mg/mL

500 mcg aliquot: transfer 0.2mL (200 µL)

GHK-Cu 50mg

Vial: 50mg lyophilised GHK-Cu

Stock solution: 5 mL solvent → 10 mg/mL

1 mg aliquot: transfer 0.1mL (100 µL)

CJC-1295 2mg

Vial: 2mg lyophilised CJC-1295

Stock solution: 2 mL solvent → 1 mg/mL

100 mcg aliquot: transfer 0.1mL (100 µL)

Frequently asked questions

How do I reconstitute lyophilised peptides?

Enter the vial contents in mg and the solvent volume in mL; the tool returns the resulting stock concentration in mg/mL. Solvent selection and all handling are determined by the receiving laboratory under its own protocols. For in vitro research only.

What units does the calculator use?

Mass in milligrams and micrograms, volume in millilitres and microlitres — 1 mg = 1000 mcg, 1 mL = 1000 µL. Both mass fields stay in sync, so you can work in whichever unit your protocol is written in.

Why does it suggest a particular solvent volume?

It favours volumes that land your target aliquot on a round, comfortably pipettable figure, typically between 50 and 500 µL. Below about 10 µL transfer accuracy falls off on most air-displacement pipettes, so the tool steers towards a larger solvent volume rather than a smaller transfer.

How long is a reconstituted solution stable?

Our safety data sheets record stability of up to 28 days at 2–8 °C in aqueous solution for most lyophilised peptides in this range, protected from light; unopened vials are typically held at −20 °C. Figures are compound-specific — the SDS tab on each product page carries that product’s own values.